Eadie-Hofstee plot

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    • #5976
      tetedupet
      Participant

      I have been told to calculate the Km of an enzme using a Eadie-Hofstee plot… however I’m not really sure. I know I need to work out the gradient of the slope, but that gives -Km… so do I just flip the signs or what?

      Thanks

    • #56518
      kiekyon
      Participant

      yes, u r right!! 😆

    • #56709
      sdekivit
      Participant
      quote tetedupet:

      I have been told to calculate the Km of an enzme using a Eadie-Hofstee plot… however I’m not really sure. I know I need to work out the gradient of the slope, but that gives -Km… so do I just flip the signs or what?

      Thanks

      i posted a post about enzyme kinetics and the different plots inclusive the mathematical background in this section. try the search option.

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