Biology Forum › Genetics › EcoRI
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- October 5, 2007 at 7:30 pm #8337kikkidParticipant
Could someone tell me if this is correct:
Explain why E. coli’s own DNA is not cut by the restriction endonuclease EcoRI?A bacterium protects its own DNA from digestion using other enzymes that modify nucleotides in the recognition site on the host DNA. E.coli makes the restriction enzyme EcoRI and the methylating enzyme EcoRI methylase. The methylase modifies EcoRI sites in the bacteria’s
own genome to prevent it from being digested. - October 6, 2007 at 12:32 am #76675raghdaParticipant
the bacterium contain both of the 2 enzyems as it uses methylases to methylate cytosine bases of bacterial DNA at restriction site , so theDNA becomes resistant to the action of the restriction endonuclease
- October 6, 2007 at 2:41 am #76677canalonParticipant
So in short your answer is correct.
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