Biology Forum Genetics EcoRI

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    • #8337
      kikkid
      Participant

      Could someone tell me if this is correct:
      Explain why E. coli’s own DNA is not cut by the restriction endonuclease EcoRI?

      A bacterium protects its own DNA from digestion using other enzymes that modify nucleotides in the recognition site on the host DNA. E.coli makes the restriction enzyme EcoRI and the methylating enzyme EcoRI methylase. The methylase modifies EcoRI sites in the bacteria’s
      own genome to prevent it from being digested.

    • #76675
      raghda
      Participant

      the bacterium contain both of the 2 enzyems as it uses methylases to methylate cytosine bases of bacterial DNA at restriction site , so theDNA becomes resistant to the action of the restriction endonuclease

    • #76677
      canalon
      Participant

      So in short your answer is correct.

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