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    • #13183
      pmngrad
      Participant

      Hi All,

      Say you have a transgenic mouse who has been genetically modified to express eGFP where ever Protein X is expressed. What events might cause eGFP not to be expressed in a cell, when you are certain that Protein X is expressed. For example, say you are looking at two cells fluorescently stained…

      1) Positive for eGFP and Positive for anti-Protein X
      2) Negative for eGFP and Positive for anti-Protein X

      I suppose that if the GFP protein was mis-folded, it would prevent the eGFP signal, however I would not expect this to happen too often. Any other ideas?

      Thank you 😕

    • #99239
      JackBean
      Participant

      I don’t think, that GFP misfolding would occur. You could have some other substance, which would adsorb the emitted light, that’s used sometimes, but for protein-protein interaction research. But I don’t think, that something like that occurred in the cell naturally 🙂

      What about some silencing?

    • #99257
      pmngrad
      Participant

      Thanks for that response – I’ll look into what silencing can do to play into this.

      I think that the actual terms I should have used for this question is:

      What would cause reduced eGFP fidelity in some cells and not others. I’m not sure if that key word triggers anyone’s brains! Thanks again!

    • #99264
      JackBean
      Participant

      well, the silencing is not the same in all cells

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