Electrophoresis

Viewing 2 reply threads
  • Author
    Posts
    • #3211
      Dr.Hawaii
      Participant

      how would raising the voltage affect the results of electrophoresis? How would lowering?

      Also how does the polarity affect electrophoresis?

    • #37260
      sdekivit
      Participant

      what is the property of the separation with electrophoresis? Molecular weight. In order to separate, you need samples with a negative charge, so polarity have only a very little contribution in the separation.

      –> when u raise the voltage, the samples will migrate faster. But you must be aware of rising of the temperature of the gel 😉

    • #37318
      Dr.Stein
      Participant

      Aee there is another Doctor here… hello 😀

Viewing 2 reply threads
  • You must be logged in to reply to this topic.