Biology Forum › Molecular Biology › Electrophoresis
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- January 15, 2006 at 12:41 am #3211
Dr.HawaiiParticipanthow would raising the voltage affect the results of electrophoresis? How would lowering?
Also how does the polarity affect electrophoresis?
- January 15, 2006 at 9:14 am #37260sdekivitParticipant
what is the property of the separation with electrophoresis? Molecular weight. In order to separate, you need samples with a negative charge, so polarity have only a very little contribution in the separation.
–> when u raise the voltage, the samples will migrate faster. But you must be aware of rising of the temperature of the gel 😉
- January 16, 2006 at 12:49 am #37318Dr.SteinParticipant
Aee there is another Doctor here… hello 😀
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