Electroporation of human bladder carcinoma 5637 cell line

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    • October 6, 2009 at 5:25 am #11947
      roniadam
      Participant

      Hi Guys,

      I am searching for the protocol of human bladder carcinoma 5637 cell line using electroporation technique. I mean what voltage, pulses and cuvette should I use for susccessful transfection? I use Genepulser machine from BioRad.
      Any help please?

    • October 6, 2009 at 2:06 pm #93382
      JackBean
      Participant

      Aren’t you following some paper?

    • October 7, 2009 at 5:28 am #93401
      roniadam
      Participant

      Hi JackBean:

      I was not able to find any paper explaining the electroporation protocol for 5637 cells. all papers I saw are transfecting the 5637 using Lipofectin or Fugene. but no paper found explaining the electroporation ptotocol.

    • October 7, 2009 at 6:21 am #93407
      JackBean
      Participant

      And you do not have any protocol from manufacturer? Are these cells electrocompetive? (able to perform electroporation)

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