I am searching for the protocol of human bladder carcinoma 5637 cell line using electroporation technique. I mean what voltage, pulses and cuvette should I use for susccessful transfection? I use Genepulser machine from BioRad.
Any help please?
I was not able to find any paper explaining the electroporation protocol for 5637 cells. all papers I saw are transfecting the 5637 using Lipofectin or Fugene. but no paper found explaining the electroporation ptotocol.