Enzyme

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    • #7855
      MIA6
      Participant

      When i read about enzyme active site and substrate, it was mentioned something about ‘enzyme concentration’? But i don’t know what that means. DOes it affect enzyme activities?
      thanks for help.

    • #74016
      Katy_Bobbles
      Participant

      The concentration (amount) of enzyme will affect enzyme-substrate binding. As enzyme concentration is doubled, the rate at which products are produced (rate of reaction) also doubles. Although this is dependent on a sufficient amount of substrate available. Likewise if substrate concentration is increased, rate of reaction also increases assuming a sufficient amount of enzyme. 🙂

    • #74256
      khenwood
      Participant

      Or in very basic terms, enzyme concentration = how much of the enzyme you have in solution.

    • #74357
      Ewa
      Participant
      quote MIA6:

      When i read about enzyme active site and substrate, it was mentioned something about ‘enzyme concentration’? But i don’t know what that means. DOes it affect enzyme activities?
      thanks for help.

      enzyme is simply a protein, so it is basically the total concentration of this specific protein you have in your solution

      however, different preparations have different activities, even if the protein concentrations are the same

      best,
      Ewa

    • #74665
      lara
      Participant

      enzyme conc effects till all the active sites are saturated with the substrates

    • #74884
      vikas srivastava
      Participant

      as far as enzymatic reactions are concerned ,enzyme concentration reflects the sense of "number of active site",which definitely affect rate of reaction,till its saturation.

    • #75106
      jtmartin
      Participant

      I’m trying to find out if anyone knows whether enzymes can be found in bones. But specifically fossils. That is, if I were to do a trace for enzymes would I find it in the bones. I’m not a biology student so I don’t know if this is a dum question.

    • #75116
      canalon
      Participant

      There are enzymes in bones, but when fossilized I do not think that any protein would still be active, if even present.

    • #75178
      jtmartin
      Participant

      Thanks Canalon and vikas for your responses on enzymes in bones. Now that I’m getting a little better understanding, tell me if I’m correct. Enzymes will continue to react as long as there are sufficient amounts of substrates to react with and sufficient amounts of enzymes. For example, if a human body intakes a number of enzymes that react a certain way, it will react until the enzymes are gone or the substrates are gone? If the substrates replenish or the enzymes replenish then the reaction can happen again?

      Does someone trace an enzyme by the reaction its creating with a substrate, or do we trace them by their molecular makeup, reaction or no reaction?

    • #75204
      mith
      Participant

      the enzymes usually won’t go away, but there will be some sort of feedback mechanism to inactivate them once the correct concentration conditions are created.

    • #75284
      MrMistery
      Participant

      the enzymes can go away too. think controlled degradation. or self-digestion by pepsin 🙂

    • #75335
      dr. dugmore
      Participant
      quote khenwood:

      Or in very basic terms, enzyme concentration = how much of the enzyme you have in solution.

      true!

    • #75358
      Qphage
      Participant
      quote jtmartin:

      I’m trying to find out if anyone knows whether enzymes can be found in bones. But specifically fossils. That is, if I were to do a trace for enzymes would I find it in the bones. I’m not a biology student so I don’t know if this is a dum question.

      I dont know which kinds of enzymes are present in bones.
      But if the protein is strong enough to resist drought conditions & extreme conditions..we can obtain it!
      The method will be same with some modifications in the protocol.

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