Enzymes- Michaelis Constant

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    • #13755
      Nicolodn
      Participant

      Would Enzyme in a dog and the same enzyme in a giraffe have the same Michaelis constant? WHY?
      THanks

    • #101247
      JackBean
      Participant

      Probably not THE SAME, but it might be pretty similar

    • #101248
      JackBean
      Participant

      Oh yeah and why… well, because they (the enzymes), probably come from the same ancestor, but have diversified a while ago, so they won’t have identical primary structure, but still it will be pretty similar (but depends on each case)

    • #101249
      Nicolodn
      Participant

      Okay, thanks. So, they will be slightly different because of differing structure?
      I thought that to, but, if they have a different structure, doesn’t that make it a different enzyme? That was what I have been confused about. Also, because the same enzyme from a different source has different optimum pH, temp,etc right?

    • #101250
      JackBean
      Participant

      I’m talking about primary structure i.e. the AAs sequence, but the 3D fold will be highly similar (maybe some loops will be missing etc.)

    • #101251
      Nicolodn
      Participant

      So, because the charges of the aa are different, then the amount of substrate able to bind to the active site will be less?

    • #101252
      JackBean
      Participant

      Well, you cannot say less, as you’re not refering to any particular enzyme 😉 But yes, it will be PROBABLY sligthly different

    • #101253
      Nicolodn
      Participant

      Sorry, could you tell me why the change in primary structure can change Km? 😕

    • #101317
      JackBean
      Participant

      Primary structure = sequence of amino acids => by changes of amino acids in the binding side you will influence the affinity and thus K_M

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