Expressing protein !! Help!

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    • #6672
      joveryant
      Participant

      Hi everyone!

      I have a big problem with expressing protein
      When i ligated my gene on vector successfully,
      and tried many different conditions
      My gene didn’t OVERexpress!!

      Now I want to use primer including my gene’s promoter
      And add some transcription factor to try to express protein

      Does it work! 😯
      Give me some suggestion please!!

    • #64553
      sachin
      Participant

      Ya; it should be………

      First you should have transfered them in first experiment…… as its comon that if you are inserting a new gene then promotor should be there with it if you need results fast…….

    • #64591
      oppox
      Participant

      one thing that may be worth looking at is the codon usage, see if u have many rare codons.

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