I have a big problem with expressing protein
When i ligated my gene on vector successfully,
and tried many different conditions
My gene didn’t OVERexpress!!
Now I want to use primer including my gene’s promoter
And add some transcription factor to try to express protein
Does it work! 😯
Give me some suggestion please!!
Ya; it should be………
First you should have transfered them in first experiment…… as its comon that if you are inserting a new gene then promotor should be there with it if you need results fast…….
one thing that may be worth looking at is the codon usage, see if u have many rare codons.