-break the cells
-get rid of cell debris
-Degrade DNA or RNA (if you want the other)
-Clean DNA and/or RNA of proteins
-concentrate and wash all unwanted reagents from the previous reactions
-Resuspend in appropriate buffer.
A million protocols using 2 few basic techniques to clean the DNA/RNA (lysis just depends on your starting material) ie Phenol chloroform and Silica/NA interactions exist.
OK. I see we have a problem here. What do you call "making" a gene.
But whatever you want to do, your steps do not really make sense to me. A short list of problems:
1/ what DNA?
2/Of what, it’s OK if it’s 1, but depends on which DNA you cut in the first place.
3/What probe? to detect what?
4/The probe is part of the Southern procedure. In fact a Southern is the use of a probe it doesn’t make sense
5/To amplify what, from which template? with which primers? And why is that coming after the Southern?