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    • #15074
      AE35
      Participant

      Hi!

      I’m new here 😀

      I hope someone can help me! I have this question:

      "When you start to grow imid resistant rice and control weeds in the field with imid, it is very efficient against socalled weedy rice, which is another type of rice (Oryza sativa). After a few years of culture, however, imid resistant types of weedy rice start to appear. How do you find out if this resistance is due ot new mutations in the weedy rice or the mutated gene from the cultivated imid resistant rice that has been transferred by hybridization?"

      What line of methods or method should be used to find this out?

      Thanks a lot!!

      Nicholas

    • #105258
      mith
      Participant

      Are you just going to post homework questions?

    • #105259
      AE35
      Participant

      Well, since I have no idea what to do, you could say that.

    • #105260
      AE35
      Participant

      In the mutation there’s a microsatellite TATATATA(this gives the resistance), that
      isn’t in the wildtype.

      Could a Southern blot be used to test for this microsat? If so, how would we
      know if it was weedy rices own mutation, or if it were from hybridization?

      Thanks!

      Nicholas

    • #108077
      JackBean
      Participant

      microsatellite by itself gives resistance?

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