• Author
Posts
• #12098
carbonp
Participant

If,
Free protein = [(Total protein – Total drug -Dissociation constant)] (+/-) under root of (Total protein – Total drug -Dissociation constant)^2 + 4 (dissociation Constant * Total Protein) divided by 2
If Total protein – 0.035 g /microliter,
Total drug – 3319.246 x 10^-15 moles/l (Molecular weight of drug – 337.41 g/mole)
Dissociation constant = 0.27 nM,
How to calculate the free protein, Above is quadratic equaltion,
As the unit dissociation constant of drug is in nM, while that of total protein and total drug is in different units, How to calculate the free protein ?

Is there any way to convert 0.27 nM in to some other unit that can be used for calculation?

• #94067
JackBean
Participant

First of all, I don’t think, you are supposed to substract the diss. const. from the protein amount ðŸ™„

• #94068
carbonp
Participant

Sorry its addition of dissociation constant. but formula does not say multiplication of dissociation constant.

• #94070
JackBean
Participant

Sorry, I don’t get it.
Anyway, it doesn’t make much sense to substract/add the dissociation constant from/to protein/drug amount