Gel Electrophoresis Help!

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    • #17505
      cks123
      Participant

      So I did a double digest for subcloning and I didn’t get two distinct bands when I ran the gel. Can someone interpret the gel pattern for me?


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    • #114268
      JackBean
      Participant

      not really, because there is hardly something visible. What sizes is it? What sizes did you expect?

    • #114270
      bravebeaker
      Participant

      size of your vector/insert.
      gel is prestained with ethidium bromide or not.
      what are the restriction enzymes you are using?
      did you check if your vector contains multiple sites of the enzymes you are using?

      The bands are way up, higher than 10k bp..

    • #114289
      Cat
      Participant

      It looks like a bad gel. I suggest to re-run with fresh buffer/gel.

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