Viewing 3 reply threads
  • Author
    Posts
    • #17402
      ghaemi100
      Participant

      Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

    • #113979
      Cat
      Participant

      The simplest is by PCR using T7 promoter primer and one of the primers you used to get your PCR product (the one that gives you desired orientation).

    • #114139
      hami
      Participant

      any body guide me in fusion protein

    • #114142
      JackBean
      Participant

      @ghaemi either PCR or restriction analysis


      @hami
      what would you like to know?

Viewing 3 reply threads
  • You must be logged in to reply to this topic.