Say I have 50mL of bacteria at an OD0.8 and I need to get bacteria to a reading of 0.1..this is what I’ve been doing in the past but just want to confirm that what I’ve been doing in correct.
So from my 50mL, I took 1mL, spun and then resuspended the pellet in 1mL of PBS. (I now have 1mL of bacteria at OD0.8 in PBS)
I took 125uL from this and added to 875uL of PBS (OD has gone down 8x now)
Is this correct–would this now give me 1mL of bacteria at an OD0.1?
Exp: Incase you want to know why I need at OD0.1 it’s because I want to add a certain about of bacteria to my assay..I know roughly how many are contained in an OD0.1 so from there I can add the correct volume ..