GFP fusion protein not expressing!

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    • June 27, 2012 at 10:19 pm #16640
      biology_06er
      Participant

      Hi there,

      I tried to make a GFP fusion protein (my gene of interest was fused to the C terminal of GFP) but no expression occured…I tested the expression of the two proteins alone and individually they worked but not when fused together..I tried a linker between the two..still no luck…could it just be that the fusion protein is somewhat toxic to the cell (doesn’t seem likely aye..especially if the 2 alones are ok)

      cheers,

      b_06er

    • June 28, 2012 at 9:35 am #111698
      JackBean
      Participant

      Try the other side of the protein or other tag. Sometimes it just doesn’t work.

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