Biology Forum › Community › General Discussion › GraphPad Prism

last updated by assaywiz 13 years, 2 months ago
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    • April 9, 2007 at 3:40 am #7392
      Dr.Stein
      Participant

      Anyone familiar with this biostatistic software? I want to have old version 3.0 , 4.0, or 4.3. Demo version will accepted. It can be used for 30 days 😆

      Thanks 😀

    • April 9, 2007 at 3:58 am #70991
      mith
      Participant

      My school recommends this freeware
      http://www.r-project.org/

    • April 9, 2007 at 5:11 am #70997
      Dr.Stein
      Participant

      Does it provide standard curve + interpolation menu and calculation for enzyme kinetics?

    • April 9, 2007 at 2:02 pm #71011
      mith
      Participant

      I have no idea lol, but it does have multidimensional distance analysis so I think it’s pretty advanced.

    • April 10, 2007 at 9:31 am #71032
      Dr.Stein
      Participant

      I am stuck with my ELISA data analysis again 🙁
      I read in its protocol that I need "Four Parameter Logistic" software to generate proper standard curve. Anyone knows how to find this software? Thank you.

    • April 10, 2007 at 12:14 pm #71036
      Vagabond
      Participant

      I believe Excel will graph it for you. As for calculating it off the graph you need an equation. I will look around I know I used to have one in a file somewhere but Now I use Prism, StatView or SoftMax

    • April 10, 2007 at 2:31 pm #71038
      CoffeaRobusta
      Participant

      What was wrong with this software that someone else told you to use?

      http://www.cdc.gov/ncidod/dbmd/bimb/elisa.htm

      I quote from the page:

      quote :

      ELISA for Windows is able to abstract the standard series, individual serum samples, and quality control samples from this file. The standards data are used to form a characteristic or standard curve which may be modeled using a three point cubic spline or a four parameter logistic-log function. The four parameters of the logistic function may be estimated using two methods: iteratively reweighted least squares and robust procedures. Estimation options include the Taylor series linearization (Gauss-Newton) and the Marquardt’s compromise estimation algorithms. The standard curve is then used to interpolate antibody concentrations for the patient isolates and quality control samples. Summary statistics are calculated from these concentrations (means, standard deviations, coefficients of variation, etc). ELISA for Windows also forms plots of the standards data with the estimated standard curve superimposed on the data points.
    • July 27, 2009 at 11:31 pm #92246
      fcs
      Participant

      I know this thread is out of date but I hope this information will reach others that are facing a similar question.

      The 4-parameter logistic model equation assumes symmetry around the inflection point whereas the 5-parameter logistic model equation takes into account the asymmetry that is present in most bioassays including elisas.

      Here is a blog post that goes into the 5-parameter logistic in more detail.

      MasterPlex ReaderFit is specifically designed for drawing standard curves and curve-fitting of unknown points from elisa assays.

      Allen
      MiraiBio

    • October 23, 2011 at 10:26 pm #107180
      assaywiz
      Participant

      Or you could use the free tools at:

      http://www.myassays.com/four-parameter-fit.assay
      http://www.myassays.com/five-parameter-fit.assay

      These are preconfigured and require no download or installation.

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