Biology Forum › Cell Biology › heat shcok protocal

last updated by mehdi71000 17 years, 7 months ago
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    • July 9, 2007 at 11:20 am #7927
      mehdi71000
      Participant

      hi can any one explain the heat shock protocol please? and can I freeze ecoli?

    • July 9, 2007 at 6:53 pm #74461
      canalon
      Participant

      Yes you can freeze E. coli, in a 15% solution of sterile glycerol.

    • July 11, 2007 at 12:33 pm #74493
      mehdi71000
      Participant

      and if its not done by glycrol will it die?

    • July 11, 2007 at 5:07 pm #74508
      canalon
      Participant

      Ice crystals will form and destroy bacteria. You might not kill all of them, but the recovery rate is not going to be good, and if you keep your cells at -18ºC, the crystals will slowly grow and cause more and more damage… But you can use DMSO (di Methyl Sulfoxide) instead.

    • July 12, 2007 at 9:28 am #74518
      mehdi71000
      Participant

      thanks mate 😀

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