I have question about the steps involved in SAGE.
So, the mRNA is bound to beads, converted to cDNA and then it’s cleaved.
For the following steps, are we working with the part of the cDNA that’s attached to the bead? or are we wroking with the released piece of cDNA after the cut (by attaching it to a sterptavidin bead)?
I’m positive that my prof. told us that we use the latter, but all the diagrams she handed out (a long with many other websites) refer to the former. So, which one is it? or can both be used?