Help with a question

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    • #16273
      bio185
      Participant

      I’m struggling with the following 2 questions. Can someone help please explain this to me? I’d really appreciate it.

      Question 1

      Two aquatic plants were grown in either water or water containing a microtubule inhibitor for 24 hours. The roots were dissected and stained for DNA. The researcher reported that in the microtubule-inhibited plant there were a much higher number of cells at metaphase.
      Describe in details what the experimenter would have observed in each root and explain the reason for the observations. What do you think would happen if the ‘inhibited’ plants were returned to plain water?

      Question 2
      You have been given an enzyme E, a substrate S and a competitive inhibitor I and you have been asked to calculate the Km of the reaction in presence and absence of the inhibitor I. However after drawing the Michaelis Menten plot you observe that even at the maximum concentration of inhibitor that you have been given, the Vmax for the inhibited reaction is lower than the un-inhibited reaction
      Describe how you would have derived the data for the Michaelis Menten plot, explain the reason why you are perplexed about the observation of the Vmax and suggest what you could do to resolve if you have been given the correct information about the nature of the inhibitor.

    • #110367
      JackBean
      Participant

      1. what happens during metaphase? How is it related to the microtubules?

      2. Are you sure there should be "that even at the maximum concentration of inhibitor that you have been given, the Vmax for the inhibited reaction is lower than the un-inhibited reaction"? Because in such question I would expect rather substrate, because with high inhibitor concentration it’s quite logic, that you won’t have as high Vmax as without inhibitor.
      The simplest answer would be insuficient inhibitor concentration, but based on last sentence, it’s probably not competitive inhibitor. However, from such vague description we can hardly say, what type of inhibitor it is.

    • #110368
      girlie21
      Participant

      YEAH BUT HOW WOULD YOU EXPLAIN THE RELATIONSHIP OF MICRO TUBULES INHIBITOR? WHAT IS THE ROLE OF MICROTUBULE??

    • #110369
      girlie21
      Participant

      I mean the role of microtubule inhibitor?*

    • #110370
      girlie21
      Participant
      quote JackBean:

      1. what happens during metaphase? How is it related to the microtubules?

      2. Are you sure there should be “that even at the maximum concentration of inhibitor that you have been given, the Vmax for the inhibited reaction is lower than the un-inhibited reaction”? Because in such question I would expect rather substrate, because with high inhibitor concentration it’s quite logic, that you won’t have as high Vmax as without inhibitor.
      The simplest answer would be insuficient inhibitor concentration, but based on last sentence, it’s probably not competitive inhibitor. However, from such vague description we can hardly say, what type of inhibitor it is.

      what is the role of micro tuble inhibitor?

    • #110371
      JackBean
      Participant

      The inhibitor probably breaks the microtubules. So, are microtubules needed for something during metaphase?

    • #110384
      cyanodave
      Participant

      Correct me if I’m wrong, but don’t the chromosomes attach to the poles of the cell via microtubules during metaphase, and are pulled by the microtubules to the poles during anaphase? if this is the case then an inhibitor would prevent the occurrence of anaphase because chromosomes would remain in the center, unable to be pulled to the sides.

    • #110393
      JackBean
      Participant

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