Help with viewing stomata method

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    • #374
      luke_j
      Participant

      Hi,

      I’m familiar with the common method used to view stomata which involves nail varnish (a google search will yield the method i’m addressing if you’re unfamilair). But i’m wondering, why is this method used? Why can’t someone simply view stomata directly using a microscope? What’s the advantage of using the nail varnish method?

      Thanks for any help

    • #19598
      mith
      Participant

      The only reason I can think of is perhaps the stomata is not rigid or will be destroyed easily when placed on a microscope. Whereas the lacquer is stronger.

    • #19666
      DevGrp
      Participant

      To view something under a high magnification microscope you need to be able to shine light through it. I expect that if you put a leaf in a microscope you wouldn’t be able to see anything. Nail varnish (the clear type) is tranparent so the light will pass straight through.

      The alternative would be to freeze it / enbed it in wax and then cut very thin sections of it. This is expensive / time consuming and it would be difficlut to get just the leaf surface.

    • #20172
      MrMistery
      Participant

      It is not neccessarly to use nail pollish. I made a prep today(strange coincidence) ago and it looks great and i didn’t use nail pollish. Here it is how i did it.
      I made a section in a leaf(if you need help with that, no pb)
      Put it into something called floroglucine and then HCL that made it pink
      put it onto the microscope
      It looks like this

      The little white spaces between the cells are the stomata, you do not see them as you should because my microscope is not so high-take. That is the maximum zoom. It was all I could affort 🙂 🙂

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