Good morning everybody,
I am currently studying the transcriptional regulation of a gene in the liver, known to be a target of HNF4α (this gene is strongly upregulated in HNF4α liver specific KO mice) in an inflammatory context (LPS).
My hypothesis is that, during inflammation, HNF4α is phosphorylated so its DNA binding activity is decreased and the transcription of my gene is not anymore inhibited by HNF4α.
So I would like to study the phosphorylation status of HNF4α in my case but I really don’t know which precise phosphorylation I need to look (serin or threonin?, localization ?).
Do you know a good antibody to do this by Western Blot ?
Thank you for your help !
Aurelie