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    • #16657
      claire84
      Participant

      Hi everyone!
      I need your help because I have little experience on cell culture techniques…I would centrifuge my Hl60 cells which are plated in a 96-well microplate, to obtain a pellet at the bottom and to remove supernatant without loss of cells.
      Could you recommend me rpm and time to use?
      I tryed with 1500 rpm for 5 minutes, but I think I need to increase the time…
      thank so much

      Chiara

    • #111875
      JackBean
      Participant

      5000 g for 1 minute is used for bacteria and I don’t think it would be much different for other cells.

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