Horse serum vs. Fetal Bovine Serum in DMEM?

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    • #5546
      curious1
      Participant

      Hi everyone,

      I was wondering if anyone knew whether it is ok to use horse serum in place of fetal bovine serum when preparing DMEM media. We usually add FBS but we ran out so I had added about 2ml of FBS and then used 23ml of horse serum to make my media. Also, is it okay to mix the two serums? Thanks in advance.

    • #55982
      Nickninja
      Participant

      It really depends on your cells, I think. We use DMEM +10% FBS +10% Horse Serum +5% penn/strep for our muscle derived stem cells. But we leave out the Horse serum for the other cells we culture.

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