How does high salt conc. influence denaturation kinetics

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    • #11843
      HelloKitty
      Participant

      How does high salt concentration influence denaturation kinetics of DNA? Explain your reasoning.

    • #93003
      Darwin420
      Participant

      Salts can change the charge of the phosphate backbone by donating electrons or possibly attracting an Oxygen atom from the phosphate ion, causing the charge to chage.. which could cause the 5′ and 3′ end to repel eachother.

    • #93011
      JackBean
      Participant

      Doesn’t he mean by denaturation just unfolding the strains one from each other?

    • #93016
      Darwin420
      Participant

      yes…and I described that. the 3′ and 5′ repel each other, thus they unfold.

      Look it up in a text.

    • #93019
      Darwin420
      Participant

      But wait, using LOW SALT concentrations is what scientist use to denature DNA. If you have high salt concentrations you will most likely have an unstable z form DNA.

      sorry, I am just learning this stuff as well…little rusty.

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