Viewing 3 reply threads
  • Author
    Posts
    • #13039
      seahow
      Participant

      I want to site-directed mutation on genome other than plasmid DNA. Anyone has protocol like this. I donnot want to introduce neo etc antibiotic maker. I need the clear genetic background which the mutation differ to the wild type with only the nucleotide.
      any help is wellcome!!
      Thank a lot.

    • #98774
      JackBean
      Participant

      what about, if you shared the species, you want to mutate? That would be a little simpler to give advice… 🙄 :-O

    • #98782
      seahow
      Participant
      quote JackBean:

      what about, if you shared the species, you want to mutate? That would be a little simpler to give advice… 🙄 :-O

      I donnot understand your advice. I mean just mutate the nucleartide with chromatin status unchanged.
      and actually I wanna research the sequence on chromatin in vivo.

      please give your advice in detail. thanks!

    • #98788
      MrMistery
      Participant

      well first you have to figure out what your target sequence is (the sequence of the chromatin you want to mutate). Then make an artificial oligo that has the mutation, and introduce it into the genome by recombination insertion.

Viewing 3 reply threads
  • You must be logged in to reply to this topic.