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    • #18036
      lch8934402
      Participant

      still now,i found that many paper mention 10%FBS+DMEM,1×105 U/liter of penicillin/streptomycin at 37°Cin
      an atmosphere of 10% CO2 and 95% relative humidity. But in some forum ,someone said,1U/ml insulin must be add into the cultrue medium.
      Does anyone tell me why,and what kind of medium shoud IEC-6 in?thank you

    • #115677
      Tricho
      Participant

      Hi,
      Sigma-Aldrich says: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS); 5% CO2

      Different people use different protocols for different experiments. Sometimes they grow fine in either, but change slightly which makes one version more suitable for your experiments. If you don’t know, just try different combinations and see which works best for you.

    • #115678
      lch8934402
      Participant
      quote Tricho:

      Hi,
      Sigma-Aldrich says: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS); 5% CO2

      Different people use different protocols for different experiments. Sometimes they grow fine in either, but change slightly which makes one version more suitable for your experiments. If you don’t know, just try different combinations and see which works best for you.

      thanks for your attation,but i still don’t konw why someone said insulin must add into the medium,otherwise,IEC-6 cells can not adhere to the flask.

    • #115686
      lch8934402
      Participant

      Normally DMEM medium is modified with 4mM L-glutamin,is that necessary to add 2mM L-glutamin into medium,and what kind of insulin should i choose,you know there are lots kinds of insulin in hospital.

    • #115695
      baranbaran
      Participant

      this a very useful data that you have posted…..well done

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