- March 26, 2015 at 4:05 pm #18036
still now,i found that many paper mention 10%FBS+DMEM,1×105 U/liter of penicillin/streptomycin at 37°Cin
an atmosphere of 10% CO2 and 95% relative humidity. But in some forum ,someone said,1U/ml insulin must be add into the cultrue medium.
Does anyone tell me why,and what kind of medium shoud IEC-6 in?thank you
- March 26, 2015 at 10:08 pm #115677TrichoParticipant
Sigma-Aldrich says: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS); 5% CO2
Different people use different protocols for different experiments. Sometimes they grow fine in either, but change slightly which makes one version more suitable for your experiments. If you don’t know, just try different combinations and see which works best for you.
- March 27, 2015 at 9:39 am #115678quote Tricho:
thanks for your attation,but i still don’t konw why someone said insulin must add into the medium,otherwise,IEC-6 cells can not adhere to the flask.
- April 8, 2015 at 2:38 am #115686
Normally DMEM medium is modified with 4mM L-glutamin,is that necessary to add 2mM L-glutamin into medium,and what kind of insulin should i choose,you know there are lots kinds of insulin in hospital.
- April 16, 2015 at 7:58 am #115695baranbaranParticipant
this a very useful data that you have posted…..well done
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