Biology Forum › Molecular Biology › How to detect proteins in my sample?
- AuthorPosts
- August 24, 2012 at 8:31 am #16772
sunhan1985Participantheard of separating them by molecular weight, and electrophoresis
What technique should I use? - August 24, 2012 at 2:45 pm #112158JackBeanParticipant
What about electrophoresis?
- September 3, 2012 at 11:40 am #112229citroenboomParticipant
Just look for SDS-PAGE. And you can combine both techniques 🙂
- October 17, 2012 at 7:50 am #112718SwapnilmicrobiologyParticipant
It depends on what is your sample.
- August 16, 2022 at 10:33 pm #219247vivian creativeParticipant
maybe you could try to detect proteins using biomarkers. based on enzyme linked-immunosorbent assay (ELISA), immunohistochemistry (IHC), and flow cytometry (FC) or other emerging protein analysis techniques such as mass spectrometry (MS).
- November 2, 2022 at 3:14 am #219355vivian creativeParticipant
Microfluidic chip has become a powerful strategy and has been utilized in many protein and proteomics researches. They offer a one-stop platform for tiny-volume sample preparation, extraction, and detection, with the benefit of reduced analysis time, improved detection limits, and high throughput.
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