how to make blood lysis buffer

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    • #16526
      karenzellie
      Participant

      Hi,

      I was wondering how I should make this recipe:

      100mM Tris HCL
      100mM EDTA
      100mM Nacl
      1%SDS

    • #111258
      JackBean
      Participant

      What’s the problem?

    • #111272
      karenzellie
      Participant

      I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?

    • #111274
      JackBean
      Participant

      You can make 1M Tris, 1M EDTA, 1M NaCl and 10% SDS, mix 100 ml of each and fill up to 1 l.

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