I was wondering how I should make this recipe:
100mM Tris HCL
What’s the problem?
I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?
You can make 1M Tris, 1M EDTA, 1M NaCl and 10% SDS, mix 100 ml of each and fill up to 1 l.