How to prepare a 3.5% agarose gel ?

Viewing 4 reply threads
  • Author
    Posts
    • #17241
      itamo
      Participant

      I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, but there were alot of bubbles in the gel.

      Is there a way to prepare a high concentration gel without the annoying bubbles?

    • #113524
      JackBean
      Participant

      quickly heat in microwave for short periods of time with mixing in between

    • #113528
      itamo
      Participant

      Thanks.

      Do you cover the flask to avoid evaporation ?
      If you do, with what ?
      Did you tried it yourself?
      How many cycles of heating and swirling did you do?

    • #113529
      JackBean
      Participant

      I usually do it in the bottle with cap and I keep it on (just not tighten it firmly). As long as it is not properly dissolved.
      I’m usually working with 1% agarose, but I think I have prepared also 4% in this way.

    • #113564
      itamo
      Participant

      I cooked it on a hot plate on low temp with a stirring magnet and in the end put it in a microwave oven on low-medium for a couple of soconds a few times to get rid of the bubbles. and after getting a clear solution i added the atidium bromide.
      worked great.

Viewing 4 reply threads
  • You must be logged in to reply to this topic.