How to read this tricky electropherogram?

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- February 21, 2015 at 3:23 am #18003
  
  kabtq9s
  Participant
  
  I am doing an exercise to find the sequence of this electropherogram and confirming the results in NCBI Blast site. I wrote the sequence on the graph to the best of my ability of ~100 bp segments and searched them in Blast… nothing came up. All what I know is that this is a human gene. Any ideas?
  
  Here are the three sequences and the actual graph
  
  lane (1)
  AAAGTGCATCTCCTCCGACATTGGAGGATCCCAAGCTCTCATGTTGCCCTTATTGTCACCAGTGACATTTATTCCAAACAGGAGTCCTTCGGGCCAGCAAA
  
  lane (2)
  GCTGCCCAGGCTTAGCTGCGAGCCCGTCATGGAGAAAAAGCTCAGAGAAAAGCAGTCTGTTGGAGAATGGGACAGTCACCAGAGACACCTCGTGGGGCTCAGCGGTTCT
  
  lane (3)
  GAACAGTACCCCGACTGGCCAGCCGAGACATGTTGACCATCCCACCCCATGCGAGCTCATCAAGGAAAGACTAAGTCAGAGAGTCCTCTCTGAC
  
  Image link
  https://mysowar.files.wordpress.com/2015/02/img.jpg
- February 23, 2015 at 3:50 pm #115664
  
  jonmoulton
  Participant
  
  Do you know if this is genomic DNA or cDNA? If not, you should BLAST against both the RNA sequences and the genomic DNA sequence.
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