Hypophysis removal - Biology Forum

last updated by

Dr.Stein 17 years, 12 months ago

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5 replies

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- January 16, 2007 at 11:49 am #6782
  Dr.Stein
  Participant
  This old lady got problem on how to remove hypophysis from its place for then to be processed for histological preparation. For your information, the animal is three-month-old white Balb/c mouse. As you may know, this strain is a tiny cuty thingy mousy 😕
  Thank you for your information 8)
  Protocol/steps or pics would be nice 🙂
- January 16, 2007 at 2:52 pm #66869
  sachin
  Participant
  Remove Hypophysis means?
- January 16, 2007 at 4:30 pm #66889
  MrMistery
  Participant
  she means remove the pituitary gland surgically…
- January 17, 2007 at 5:45 am #67016
  Dr.Stein
  Participant
  Andrei is correct. Because the gland is a tiny baby, super care must be taken to remove it out. However, when the removal is successful, we still need to process for preparation. This means we will face another problem because organs/tissues sometime get shrinked in fixative or another chemicals in the process. I cannot imagine if that tiny baby will be shrinked. I will get nothing to examine by then 🙁
- January 17, 2007 at 2:06 pm #67052
  sachin
  Participant
  That’s the major problem most of the time….
  But can you tell which method you are using currently for that; may be we can help you….
- January 18, 2007 at 9:31 am #67193
  Dr.Stein
  Participant
  Routine histological method: paraffin method with fixative formaldehyde and standars PAS staining for beta cell.
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