IGG1K WESTERN BLOT

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    • #18567
      Biologos
      Participant

      Dear all,

      i an trying to detect an Ab (Igg1k) using an anti-igg1 ab from thermo. When i use dtt or mercaptoethanol i get a single band of either 72-75 kda or around 50 kda. Do i get a breakdown of 1 heavy and 1 light chain (75kda) x2? Or do i only detect heavy chains (around 50kda)?

      Your help will be highly appreciated.

      Thank you.

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