interpreting sequence alignment

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    • #11249
      helpmeplz
      Participant

      http://upload.wikimedia.org/wikipedia/c … nment2.png

      say you have a sequence like that^, how do you interpret it?

      i think only the first line is an open reading frame
      the second and third line has ‘gaps’
      4th line is an open reading frame.

      is that it? is that all it tells you, whether its an open reading frame or not?

      what eslse am i meant to be seeing??

      please help..i’m panicing

    • #90784
      smellor
      Participant

      think about what a sequence alignment is…
      in terms of looking for ORF’s, you would probably use a tool like Expasy for that, sequence alignments serve a different purpose.
      Assuming line 1 and 3 are continuous, as are 2 and 4, what do they tell you?
      what does asterisk signify, do you think. what does : signify (think about amino acids E and Q), what does . signify (think about G and S)…

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