- October 22, 2011 at 1:01 pm #15571SWEngineerParticipant
Do we sterilize a wire loop by putting it in a flame until it becomes red?
- October 22, 2011 at 2:39 pm #107120JackBeanParticipant
are you cheking our knowledge?
- October 24, 2011 at 3:30 am #107200daniel.kurzParticipant
Guessing from the number of posts by that user that are all very basic, the person is not in microbiology. No you do not need to heat it till it becomes red. A short duration ~1 second to be absolutely certain will suffice. The microbes are killed rapidly by the quick change from cold to hot to relative cold. The shock kills them. Kinda like taking a human to volcanic lava and then to the Greenland ice shelf.
- October 26, 2011 at 1:53 am #107299canalonParticipant
Good practice in my microbio lab is to leave it until red. Particularly useful if you have clumps that are attached, a simple heating will not kill all bacteria. And I ‘d rather do a bit of overkill, than having to redo an experiment… Just saying.
- October 26, 2011 at 3:26 pm #107318JackBeanParticipant
agree with canalon
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