kovacs reagent

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    • #16052
      michimichi
      Participant

      1. what is the storage temp.for prepared Kovacs reagent
      2. can i add the Kovacs reagent directly to positive Lauryl Tryptose Broth to detect e.coli or do i have to use Tryptophan broth?

    • #109498
      canalon
      Participant

      1. We used to put it at 4°C, and never had problems
      2. You have to use tryptophan broth to avoid false negative.

    • #109500
      michimichi
      Participant

      can an indole test (kovacs) alone can prove that a water sample has e.coli ?
      about the storage of kovacs – i stored it at room temp , so it isnt acceptable.?
      can i use peptone water as a subtitute for tryptophan? 🙂

    • #109508
      canalon
      Participant

      No.
      Don’t know, but considering the ingredients, I imagine that the problem is more the stability of the coloring agent. It might be OK.
      That is what I used. Peptone broth has enough aa including tryptophane that it was considered as a good substrate for indole reaction.

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