can an indole test (kovacs) alone can prove that a water sample has e.coli ?
about the storage of kovacs – i stored it at room temp , so it isnt acceptable.?
can i use peptone water as a subtitute for tryptophan? 🙂
Don’t know, but considering the ingredients, I imagine that the problem is more the stability of the coloring agent. It might be OK.
That is what I used. Peptone broth has enough aa including tryptophane that it was considered as a good substrate for indole reaction.