February 1, 2007 at 11:54 am #6884vergil5203Participant
As i know
Oxaloacetate>(combine with acetyl CoA)>citrate>>iso-citrate>>alpha-ketogutarate>>sucinyl coA>>sucinate>>fumalate>>malate
I wan to know…what are substance is it..i just know all of it is Acid…erm..and which is enzyme n which group of protein? Conjugated? simple? phosphetic? or ??
February 1, 2007 at 5:44 pm #68479
February 8, 2007 at 11:00 am #68730
So how can we know the acids is in the cycle and how can we located in the cycle?
that’s my question…
February 8, 2007 at 8:16 pm #68746sdekivitParticipant
don’t understand the question.
February 9, 2007 at 1:45 am #68753
i want to ask the cycle how can be found in the past?Can u get the point now?
thank u very much though,sdekivit.
February 9, 2007 at 2:34 pm #68785
You want to know how the cycle was elucidated?
February 9, 2007 at 6:03 pm #68797
that’s really trouble me for a long time .
please tell me about that,thanks in advance.
February 9, 2007 at 7:35 pm #68806
i may be wrong, but as far as i remember Hans Krebs and his team used some radioactive carbon to track the CO2 that came from acetyl~CoA. By bursting cells open at various times he found the Carbon atoms in various components at different times after marking. That led to finding the intermediates and the order which they are formed in. The detail were worked out later..
February 10, 2007 at 12:27 pm #68823UltrashogunParticipant
Im pretty sure Krebs and his team uncovered the cycle by adding intermediates of the cycle to the system and noticing how this affected the concentrations of the other intermediates.
February 10, 2007 at 2:21 pm #68827
so they could ensure about the sequence of the intermediates.
i think i get it now.thanks
February 15, 2007 at 8:23 am #68949vergil5203Participant
erm…ic…i more understand more.However,i wan noe a substance means.Coenzyme or a enzyme for oxaloacetate n following name..
February 15, 2007 at 3:56 pm #68969
February 16, 2007 at 4:30 pm #69040CyLMHParticipant
Dunnt know if u r still interested in this question.
the TCA circle was proprosed by Krebs. but actually, the specific research of the mechanisms were done by other scienstists such as Maritus & Knoop who showed that citrate could be formed nonenzymatically from OAA and pyruvate with H2O2 in basic medium. it was already "common sense" to the scienstists at that time that the cirtrate->isocitrate->alpha-ketoglutarate->succinate->fumarate->malate->OAA.
But a small amount of any of the intermediate would result in increasing the total reaction significantly. So Krebs proprosed the TCA cycle by closing the rxn: pyruvate + OAA->citrate + CO2
that is what i learnt from a gentle professor from India. Guess you may find almost the same info from the text book. (any biochemistry book will give detailed info on it)
hope this will do a little for you.
February 17, 2007 at 3:36 pm #69084dipjyotiParticipant
What do you exactly want to know, Krebs Cycle or any thing else? Use wikipedia or google.
Dip Jyoti Chakraborty
February 22, 2007 at 3:52 pm #69317
i am reading the lehninger’s book of biochemistry for the detail.i think it may helpfull.
i want to know his experiment he has taken for elucidation.
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