Krebs Cycle

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    • #6884
      vergil5203
      Participant

      erm..

      As i know

      Oxaloacetate>(combine with acetyl CoA)>citrate>>iso-citrate>>alpha-ketogutarate>>sucinyl coA>>sucinate>>fumalate>>malate

      I wan to know…what are substance is it..i just know all of it is Acid…erm..and which is enzyme n which group of protein? Conjugated? simple? phosphetic? or ??

    • #68479
      sachin
      Participant

      http://wine1.sb.fsu.edu/krebs/krebs.htm

      This will definately help you for all….

    • #68730
      whirlboy
      Participant

      So how can we know the acids is in the cycle and how can we located in the cycle?
      that’s my question…

    • #68746
      sdekivit
      Participant

      don’t understand the question.

    • #68753
      whirlboy
      Participant

      sorry,
      i want to ask the cycle how can be found in the past?Can u get the point now?
      thank u very much though,sdekivit.

    • #68785
      MrMistery
      Participant

      You want to know how the cycle was elucidated?

    • #68797
      whirlboy
      Participant

      oh,yes.
      that’s really trouble me for a long time .
      please tell me about that,thanks in advance.

    • #68806
      MrMistery
      Participant

      i may be wrong, but as far as i remember Hans Krebs and his team used some radioactive carbon to track the CO2 that came from acetyl~CoA. By bursting cells open at various times he found the Carbon atoms in various components at different times after marking. That led to finding the intermediates and the order which they are formed in. The detail were worked out later..

    • #68823
      Ultrashogun
      Participant

      Im pretty sure Krebs and his team uncovered the cycle by adding intermediates of the cycle to the system and noticing how this affected the concentrations of the other intermediates.

    • #68827
      whirlboy
      Participant

      so they could ensure about the sequence of the intermediates.
      i think i get it now.thanks

    • #68949
      vergil5203
      Participant

      erm…ic…i more understand more.However,i wan noe a substance means.Coenzyme or a enzyme for oxaloacetate n following name..

    • #68969
      MrMistery
      Participant

      What?

    • #69040
      CyLMH
      Participant

      Dunnt know if u r still interested in this question.
      the TCA circle was proprosed by Krebs. but actually, the specific research of the mechanisms were done by other scienstists such as Maritus & Knoop who showed that citrate could be formed nonenzymatically from OAA and pyruvate with H2O2 in basic medium. it was already "common sense" to the scienstists at that time that the cirtrate->isocitrate->alpha-ketoglutarate->succinate->fumarate->malate->OAA.
      But a small amount of any of the intermediate would result in increasing the total reaction significantly. So Krebs proprosed the TCA cycle by closing the rxn: pyruvate + OAA->citrate + CO2
      that is what i learnt from a gentle professor from India. Guess you may find almost the same info from the text book. (any biochemistry book will give detailed info on it)
      hope this will do a little for you.

    • #69084
      dipjyoti
      Participant

      So!
      What do you exactly want to know, Krebs Cycle or any thing else? Use wikipedia or google.
      Thank You.
      Dip Jyoti Chakraborty

    • #69317
      whirlboy
      Participant

      i am reading the lehninger’s book of biochemistry for the detail.i think it may helpfull.
      i want to know his experiment he has taken for elucidation.

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