Hi, I have been reading a paper, and understand the aims and outcomes of the study, but don’t understand the methods they use. Why do they use these techniques?
Here’s a link: http://onlinelibrary.wiley.com/doi/10.1 … 7049.x/pdf
Here’s the reference:
Maiso, P. et al. 2007. The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells. British Journal of Haematology. 141, 470–482
Here’s my (patchy) interpretation: Cell proliferation, cell cycle and apoptosis assays- They are analysing the normal growth of the MM cells as a control
Microarray RNA analyses- To find out when the beginning of apoptosis is, and extract the total RNA at this point-but don’t know why
Immunoprecipitation and Western blotting- not sure what they’re trying to extract and analyse here.
Subcellular fractionation- Again, not too sure here.
Effect of interleukin-6, IGF-I and BMSC on NVP-AEW541-induced growth inhibition- This is to see what effect these chemicals have on NVP-AEW541
In vitro kinase assay- an in vitro test on the effects of NVP-AEW541 on the MM cells
MM xenografts- An in vivo test on a mouse
Any help on explaining the methodology of this paper would be very helpful- Thanks!