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    • #14600
      lrob
      Participant

      Hi, I have been reading a paper, and understand the aims and outcomes of the study, but don’t understand the methods they use. Why do they use these techniques?
      Here’s a link:
      http://onlinelibrary.wiley.com/doi/10.1 … 7049.x/pdf

      Here’s the reference:
      Maiso, P. et al. 2007. The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells. British Journal of Haematology. 141, 470–482

      Here’s my (patchy) interpretation:
      Cell proliferation, cell cycle and apoptosis assays- They are analysing the normal growth of the MM cells as a control

      Microarray RNA analyses- To find out when the beginning of apoptosis is, and extract the total RNA at this point-but don’t know why

      Immunoprecipitation and Western blotting- not sure what they’re trying to extract and analyse here.

      Subcellular fractionation- Again, not too sure here.

      Effect of interleukin-6, IGF-I and BMSC on NVP-AEW541-induced growth inhibition- This is to see what effect these chemicals have on NVP-AEW541

      In vitro kinase assay- an in vitro test on the effects of NVP-AEW541 on the MM cells

      MM xenografts- An in vivo test on a mouse

      Any help on explaining the methodology of this paper would be very helpful- Thanks!

    • #103776
      JackBean
      Participant

      you should read the results, there you should find, which experiment was used to explore which part.

      blots: look e.g. on Fig. 3, there you find, what were they blotting…

      fractionations – they were probably interested in which organelle are some of their proteins. But again look ito that article

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