I am a new member 😀 and I have A question which I would like to share it with you to help me to get the reason.
I am asking why do I got double band align under each other in Agarose Gel when I load DNA PCR Product ???
Knowing that this DNA is Human DNA and I got it from Buccel swab and I am working in SNPs sequencing.
Yes, I check all of what you said and It works at the first time without any problems, just suddenly it appeared without changing any things except I increased No. of cycling to 35 instead of 30 so is that a big deal and may appear as a double band in Agarose Gel ???