Loading control for different human tissue samples

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    • #13097
      samkh918
      Participant

      Hello,

      I have ordered an immunoblot (NC membrane) for western blotting, which contains different human tissues such as kidney, spleen, prostate, etc. in each lane. I want to see if equal amount of proteins have been loaded in each lane as promised by the company but my problem is that I can’t use B-tubulin as the loading control since different tissue express different levels of this protein.
      I was wondering what would be the best way for this.

      Thank you in advance for your reply.

    • #99119
      MrMistery
      Participant

      So wait someone else ran a gel and blotted it, and then just sent you the membrane? Jesus, what would they think of next?
      Anyway, maybe PGK? Actin?

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