Hello,
I have ordered an immunoblot (NC membrane) for western blotting, which contains different human tissues such as kidney, spleen, prostate, etc. in each lane. I want to see if equal amount of proteins have been loaded in each lane as promised by the company but my problem is that I can’t use B-tubulin as the loading control since different tissue express different levels of this protein.
I was wondering what would be the best way for this.
Thank you in advance for your reply.
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