low levels of protein but high activity levels

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    • #17012
      sns
      Participant

      Hi,
      I am stumped on one of my projects. In my experiment I found that the gene expression is high, protein levels low but activity levels are high for this enzyme in diseased samples compared to age-matched controls. Has anyone else seen anything like this?

    • #112902
      JackBean
      Participant

      No, nobody.

      Now seriously. Do you know what is the specific activity of your pure enzyme? That could tell you something. How do you determine the protein level?

    • #112954
      sns
      Participant

      I determined the protein levels using westerns

    • #112956
      JackBean
      Participant

      the antibody was against tag or your protein?

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