MCAC - Biology Forum

last updated by

JackBean 13 years, 2 months ago

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- October 11, 2011 at 2:01 am #15499
  biology_06er
  Participant
  Hi there,
  Regarding protein purification I have to make up buffers
  MCAC-0: 20mM Tris.HCl, pH7.9
  0.5 M NaCl
  10% glycerol
  MCAC-1000: 20mM Tris.HCl, pH7.9
  0.5 M NaCl
  10% glycerol
  1M imidazole
  what does MCAC-0, 1000 mean. What does the number after it mean?
- October 11, 2011 at 5:56 am #106802
  JackBean
  Participant
  what’s the only difference between these two solutions? The number is obviously concentration of imidazole in mM
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