micromolar conversion

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    • #13179
      roniadam
      Participant

      Hi All

      I am getting stuck

      My primer concentration is 0.1 uM in 10 uL and I need to get 125 ng of this primer.
      how many uL should i take from my primer in total reaction of 50 uL.

      Is there any online calculator that can help me ???

    • #99206
      JackBean
      Participant

      calculator? 😆

      These are simple chemical calculations, I guess you should know them, if you’re doing PCR.

      m = n.M
      c = n/V

    • #99219
      jonmoulton
      Participant

      You have a concentration in micromoles. You want to deliver a particular number of nanograms. First convert micromolar (which is micromoles/liter) to nanograms/liter. To do that you will need the molecular mass of your primer. Watch out for the prefixes, it is easy to mess up the calculations by incorrectly converting micro to nano. Once you have converted micromolar to nanograms/liter, determine how many microliters you need to get your desired number of nanograms.

    • #99267
      roniadam
      Participant

      Hi
      Thank you very much for your kine help

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