Can anyone please tell me if microRNAs are isolated along with larger RNA molecules using the caesium chloride RNA extraction protocol?
I have previously extracted RNA from frozen cell line material using caesium chloride centrifugation. I am now interested in microRNA and was wondering if my previously extracted RNA would also contain these small molecules.
There is an active miRNA research subforum on BioForum. You might try your question there. I can’t help with miRNA extraction steps, but they are often discussed in the siRNA, microRNA and RNAi subforum linked below.