microRNA Isolation

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    • #12691
      Labgrl
      Participant

      Can anyone please tell me if microRNAs are isolated along with larger RNA molecules using the caesium chloride RNA extraction protocol?

      I have previously extracted RNA from frozen cell line material using caesium chloride centrifugation. I am now interested in microRNA and was wondering if my previously extracted RNA would also contain these small molecules.

    • #97177
      jonmoulton
      Participant

      Labgrl,

      There is an active miRNA research subforum on BioForum. You might try your question there. I can’t help with miRNA extraction steps, but they are often discussed in the siRNA, microRNA and RNAi subforum linked below.

      http://www.protocol-online.org/forums/index.php?showforum=21

      Regards,

      – Jon

    • #97796
      microRNAmiRNA
      Participant

      FOR miRNA microRNA ISOLATION SEE PUBLICATION

      MicroRNA isolation and stability in stored RNA samples.
      Mraz M, Malinova K, Mayer J, Pospisilova S.
      Biochem Biophys Res Commun. 2009 Dec 4;390(1):1-4. Epub 2009 Sep 19. Review.

    • #97797
      JackBean
      Participant

      Nekřič na nás! To spamuješ s vlastním článkem?

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