more information Acrilamide vs agarose

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    • #10396
      alessandra
      Participant

      Hi I need some help

      Someone can tell me about a book, article, where I can find the best use of acrylamide and agarose gel. I mean acrilamide gives high resolution, but I couldn’t find more information about this.
      thanks…

    • #87015
      canalon
      Participant

      What do you want know?
      Wikipedia in electrophoresis will probably give you a good answer but in short:
      -Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).
      -Agarose is not as discrimant, but is good enough to discriminate samples with 20bp or more difference (depending on the size), easy to prepare, and harmless until stained. And cheaper to prepare.

    • #87027
      Bionic
      Participant

      my teacher ever said to me.. depend on the object which our learned if we study abot DNA we use agarose and if protein or enzyme is acrylamide

    • #87053
      canalon
      Participant
      quote Bionic:

      my teacher ever said to me.. depend on the object which our learned if we study abot DNA we use agarose and if protein or enzyme is acrylamide

      Although When working with DNA, agarose is used most of the time, for some applications, acrylamide is used. For example to run your gel in highly denaturing conditions (TGGE, DGGE), or to look at very short difference in size (sequencing)

    • #87073
      MrMistery
      Participant
      quote canalon:

      -Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).

      Or you can just buy ready-cast 😆

    • #87094
      canalon
      Participant
      quote MrMistery:

      quote canalon:

      -Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).

      Or you can just buy ready-cast 😆

      If you can afford it. Or if they fit your needs… But even agarose exist ready cast now… and some even include the EtBr. Good for those who can afford that kind of thing.

    • #87096
      MrMistery
      Participant
      quote canalon:

      If you can afford it.

      That’s why I love Harvard 😆 😆

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