Really need some help. I need to know how the later differentiation of different types of motor neuron takes place and an experiment that can be used to investigate the effects of different target muscles on the properties of the motor neurons that innervate them, a property of the neuron that could be investigated iit and the technique to determine it.
I know how early differentiation occurs through Shh which stimulate neural precursor cells to express different combinations of regulatory genes. But what happens from there on? And the experiment part i really dont have a clue.