- August 23, 2005 at 7:45 am #1729
how a mtDNA exract appears on agarose gel , as one band parallal to the expected size of a ladder marker. dose a mitochondrial pellet differ in colour according to the organism ???? i ve been searching for answers for six monthes now .can anybody help 😕
- August 23, 2005 at 11:13 pm #28807Daniel TillettParticipant
I don’t want to be harsh, but you do realise that mitochondria do vary in size depending on the organism.
To answer your question about the colour of the mitochondrial DNA, no it should not vary in colour depending on the organism (all pure DNA is colourless). However, if your DNA is contaminated with proteins and other junk then you can get a coloured pellet.
- August 24, 2005 at 9:11 am #28811
[i think there is a missunderstanding here ,u see ,never mind the mtDNA genome size differance i still didnt get my answer. 🙂 ,lets say that i extracted human mtDNA ,the expected size would be like 16.56bp.now do i expect this to appear as a band parallel to a similar band size in a ladder marker or it would appear near the well? that was my qustion.
also i know how DNA looks,But again that was not my qustion,i asked about the mitochondrial PELLET color not miotochondrial DNA color
- August 24, 2005 at 10:55 am #28812Daniel TillettParticipant
Mitochondria DNA is circular. How it runs on a agarose gel will depend on a number of factors:
1. Agarose %
2. Presence or absence of EtBr
3. Level of supercoiling of the DNA
So the simple answer is no. If you want to size your mitochondrial DNA digest with a couple of different RE and add up the bands.
I think I did answer your question about the pellet- if it is dirty it may be coloured and if so the colour will depend on what coloured contaminates are present in the organism.
- August 25, 2005 at 5:39 pm #28848
Well, thanks for the info.this is pretty beneficial,
i would like to go for theoretical questions like if I’m to do a search on certain mtDNA regions that are more susceptible to mutations, would i go for the conserved regions or other ones?
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