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    • #1729
      rawa zahid
      Participant

      how a mtDNA exract appears on agarose gel , as one band parallal to the expected size of a ladder marker. dose a mitochondrial pellet differ in colour according to the organism ???? i ve been searching for answers for six monthes now .can anybody help 😕

    • #28807
      Daniel Tillett
      Participant

      I don’t want to be harsh, but you do realise that mitochondria do vary in size depending on the organism.

      To answer your question about the colour of the mitochondrial DNA, no it should not vary in colour depending on the organism (all pure DNA is colourless). However, if your DNA is contaminated with proteins and other junk then you can get a coloured pellet.

      Daniel

      PCR sequencing reagents

    • #28811
      rawa zahid
      Participant

      [i think there is a missunderstanding here ,u see ,never mind the mtDNA genome size differance i still didnt get my answer. 🙂 ,lets say that i extracted human mtDNA ,the expected size would be like 16.56bp.now do i expect this to appear as a band parallel to a similar band size in a ladder marker or it would appear near the well? that was my qustion.
      also i know how DNA looks,But again that was not my qustion,i asked about the mitochondrial PELLET color not miotochondrial DNA color

    • #28812
      Daniel Tillett
      Participant

      Mitochondria DNA is circular. How it runs on a agarose gel will depend on a number of factors:

      1. Agarose %
      2. Presence or absence of EtBr
      3. Level of supercoiling of the DNA

      So the simple answer is no. If you want to size your mitochondrial DNA digest with a couple of different RE and add up the bands.

      I think I did answer your question about the pellet- if it is dirty it may be coloured and if so the colour will depend on what coloured contaminates are present in the organism.

      Daniel

      Better DNA sequencing results

    • #28848
      rawa zahid
      Participant

      Well, thanks for the info.this is pretty beneficial,
      i would like to go for theoretical questions like if I’m to do a search on certain mtDNA regions that are more susceptible to mutations, would i go for the conserved regions or other ones?

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