Mutants and knockdowns differ in compensation response

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    • #18080
      jonmoulton
      Participant

      If you want to knock down a gene to learn about its function, it can be useful to assess the loss of function without a background of altered gene expression due to compensation. Now there are data showing that mutants can compensate and the response differs from that of morphants.

      Rossi A, Kontarakis Z, Gerri C, Nolte H, Hölper S, Krüger M, Stainier DYR. Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature. 2015;[Epub ahead of print] doi:10.1038/nature14580
      http://www.nature.com/nature/journal/vaop/ncurrent/full/nature14580.html

      Hesman Saey T. Mutation-disease link masked in zebrafish. ScienceNews. 2015;July 13
      https://www.sciencenews.org/article/mutation-disease-link-masked-zebrafish

    • #115740
      bocsciences2005
      Participant

      Nice suggestion to learn about the function of a gene. By the way you suggested, the learning process will be more interesting and there will be more possibilities for the discovery of unknown. Thanks for the sharing!-BOC Sciences Screening Libraries ( http://www.bocsci.com/Screening-Libraries.asp )

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