April 2, 2006 at 5:33 am #4282NithinParticipant
How to induce mutation in yeast and keep it say alive??
I think by TRANSFORMATION (Correct me if I am wrong)
I have heard that the genome of yeast has a large number of cancerous genes which, when mutated turn malignant and kill the yeast cell. I do not know if this is true??
Now as far as I know, mutation in a non cancerous sequence of DNA or RNA can also be lethal. Now in order to avoid this there are several repair mechanisms. How do the repair mechanisms of the yeast work???
I would like to know a specific example where a mutagen causes mutation in some other organism (say Drosophila) and not yeast because of the presence of DNA repair (mismatch repair) mechanism in yeast which causes reversion from the mutated sequence to the original type.
I want to know the function of yeast’s DNA repair mechanism with respect to a specific mutagen??
I think that I am the most confused person on earth regarding this. Hope I did not confuse you too!!! 😮
I have come across something called "WILT". I think it has something to do with sequences susceptible to mutation. What does WILT sand for anyway??
April 2, 2006 at 7:21 am #448762810712Participant
So yeast species is still surviving with those Preoncogenes… so u say that their repair mechanism must be efficient and sensitive enough…
And to have the yeast cell die of cancer due to mutation in 1 or many of those pe-oncogenes it should remain alive even after mutation… and transformation is according to you the way it can be done, right?
when i asked my teacher why don’t we use transformation as a vector of a gene in recombinant DNA tech. they told me that its efficiency is less , in the sence most of DNA gets wasted… but the yeast will survive to die of the cancer though.This can be a way to induce the desired carcinogen in it…while radiation and mutagen chemicals don’t necessarily induce the ‘desired’ carcinogenic mutation… so this is ur trick… nice i’m interested in the results too… we have insert many cells from many diff. origins [ yeast, bacteria, algae, humans, plants, goat, mouse , annelids etc.] and we can then talk about how the’ efficiency’ of that carcinoggenic gene varies among these… the we can get into detail for specific mechanism mapping for each important and representative case…
By the way, why do you want it? seems you want to induce that anti-cancer mechanism in humans and cattle…how would you do that…depends upon the mechanism.. but it seems much hard to induce a mechanism…u can just use their genes to get produce their repairing prteins [ if they work on humans and cattle] and then insert the proteins in humans and cattle tissue as ‘medicine’…
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