N/C terminus - Biology Forum

last updated by

biology_06er 12 years, 11 months ago

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- December 4, 2011 at 5:56 am #15789
  biology_06er
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  Hi there,
  I recently cloned my gene of interest into a Pet32a vector containing a GFP protein..sites BgII/EcoR1. I then fused my gene containing EcoR1/Xho1 sites. When I look at the Pet vector map, am I right in saying my gene is cloned downstream of GFP and attached to the C TERMINUS of GFP.
  cheers,
  b_06er
  N.B…this is the map I am looking at (I can’t seem to attach the file hence the web link)..pet32a-c
  http://www.merck-chemicals.co.uk/life-s … ed+Kingdom
- December 4, 2011 at 6:05 am #108563
  biology_06er
  Participant
  oh and would my thioredoxin tag be on the N terminal of GFP/any gene cloned into the MCS
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