i’ve an unpublish sequence of the coat protein of banana mild mosaic virus. i hv tried some primers but it doesnt give me the sequence that i wan. hope to get some advice in dsigning primers for my sequence.
its hard to know where to start without seeing the gene. the forward primer should be located in the beginning and the reverse in te end of the gene. thats pretty much it.
Its hard to say anything but that right know since I dont know what u are going to do with it, are u having primers with a restriction site?
what sytem are u going to put it in?
also u can check up on touchdown PCR if u get unspecific binding.
If your primers contain to much AT u can add some GC.